Journal Articles

Worldwide Impact of Globodera

A qRT-PCR method to evaluate viability of potato cyst nematode (Globodera spp.)

 

  Benjamin Mimee; Brahim Soufiane; Nathalie Dauphinais; Guy Bélair

One of the main challenges to PCN management is the ability of PCN to remain dormant in the soil for several decades. For that reason, many countries have strict quarantine regulations for PCN. These regulations, although expensive and restrictive for growers, are necessary to prevent further spread of PCN but should be lifted when no more viable cysts are found. Here, we report a promising qRT-PCR method for the quantification of viable eggs and propose that this method be included in routine testing. The method was successful for quantifying G. ellingtonae, G. rostochiensis and G. pallida and was found to be very sensitive with the systematic detection of a single larva.

Citation: Mimee, Bl, B. Soufiane, N. Dauphinais, and G. Bélair. 2017. A qRT-PCR method to evaluate viability of potato cyst nematode (Globodera spp.). Canadian Journal of Plant Pathology. http://dx.doi.org/10.1080/07060661.2017.1382574


Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida

 

  Marc-Olivier Duceppe ; Joël Lafond-Lapalme; Juan Emilio Palomares-Rius; Michaël Sabeh; Vivian Blok; Peter Moffet; Benjamin Mimee

Citation: Duceppe, M.-O., J. Lafond-Lapalme, J. E. Palomares-Rius, M. Sabeh, V. Blok, P. Moffett, and B. Mimee. 2017. Analysis of survival and hatching transcriptomes from potato cyst nematodes, Globodera rostochiensis and G. pallida. Scientific Reports 7, Article number: 3882. doi:10.1038/s41598-017-03871-x


Draft Transcriptome of Globodera ellingtonae

 

  Wendy S. Phillips; Sebastian Eves-Van Den Akker, and Inga A. Zasada.

Globodera ellingtonae is a newly described cyst nematode found in Idaho, Oregon, and Argentina.  Here we present the first transcriptome assembly of G. ellingtonae, providing a valuable resource for comparing the evolution of expressed genes between potato cyst nematode species. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507131/

Phillips, W. S., Eves-van den Akker, S., & Zasada, I. A. (2017). Draft Transcriptome of Globodera ellingtonaeJournal of Nematology49(2), 129–130.


The Draft Genome of Globodera ellingtonae

 

  Wendy S. Phillips; Danna K. Howe; Amanda M.V. Brown; Sebastian Eves-Van Den Akker; Levi Dettwyler; Amy B. Peetz; Dee R. Denver; and Inga A. Zasada

Globodera ellingtonae is a newly described potato cyst nematode (PCN) found in Idaho, Oregon, and Argentina.  Here, we present a genome assembly for G. ellingtonae, a relative of the quarantine nematodes G. pallida and G. rostochiensis, produced using data from Illumina and Pacific Biosciences DNA sequencing technologies. 

Phillips, W. S., Howe, D. K., Brown, A. M. V., Eves-van den Akker, S., Dettwyler, L., Peetz, A. B., Denver, R.E., Zasada, I. A. (2017). The Draft Genome of Globodera ellingtonaeJournal of Nematology49(2), 127–128. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5507130/


A new method for decontamination of de novo transcriptomes using a hierarchical clustering algorithm

 

  Joël Lafond-Lapalme; Marc-Olivier Duceppe; Shengrui Wang; Peter Moffett; Benjamin Mimee

Citation: Lafond-Lapalme, J., M.-O. Duceppe, S. Wang, P. Moffett and B. Mimee. 2016. A new method for decontamination of de novo transcriptome using a hierarchical clustering algorithm. Bioinformatics. doi: 10.1093/bioinformatics/btw793


The genome of the yellow potato cyst nematode, Globodera rostochiensis, reveals insights into the genomic and transcriptomic bases of pathogenicity and virulence.

 

 

Eves-van den Akker, S., D.R. Laetsch, P. Thorpe, C.J. Lilley, E.G.J. Danchin, M. Da Rocha, C. Rancurel, N.E. Holroyd, J.A. Cotton, E. Grenier, J. Montarry, B. Mimee, M.-O. Duceppe, I. Boyes, J. Lapalme, M. Esquibet, M. Sabeh, M. Rott, H. Overmars, A. Tomczak, G. Smant, G. Koutsovoulos, V. Blok, S. Mantelin, P.J.A. Cock, W. Phillips, J. Marvin, L.M. Jones, H.B. Yusup, P.E. Urwin, M. Blaxter and J.T. Jones. 2016. The genome of the yellow potato cyst nematode, Globodera rostochiensis, reveals insights into the genomic and transcriptomic bases of pathogenicity and virulence. Genome Biology 17:124. DOI: 10.1186/s13059-016-0985-1.